Amount of generators of reactive oxygen species as a marker of organism damage on the background of experimental limb ischemia-reperfusion

Massive blood loss resulting in hemorrhagic shock requires the use of a hemostatic tourniquet. According to literary sourced using tourniquet for 2 hours is safe, but newest investigations have shown ambiguous consequences body, coused by both local changes tissues under tourniquet’s pressure, and outflow metabolic products into general bloodstream from primarly ischemic limb.The aim was identify interralation between level production reactive oxygen species neutrophils peripheral due ischemia-reperfusion limb, as well its combination with volumetric or mechanictal trauma.Materials methods. The experiment perfomed on 260 white male rats (200-250 g), which were divided 5 groups: control, EG-1 – simulation isolated limb (IR), EG-2 loss, EG -3 IR EG-4 modeling thigh mechanical injury, EG-5 injury. percentage neutrophils-producers (ROS) determined flow cytofluometry.Results. It found that acute massive their led an increase number ROS producers all study periods. In 1st 3rd days it higher than KG 2,41 times 2,39 accordingly, also 7th day exceeded remained raised 14th . EG-3 after injury CG data 2,5 times, then slightly decreased 2,3 day. However, index 2,6 3,3 times. peculiarities indicate effect mutual burden statistically significantly compared 3 63,7%. Also, day, 2,4 EG-1, 58,3% EG-4. this case, only figure increased till beame close initial rate.